Evidence for Sodium Dodecyl Sulfate/Protein Complexes Adopting a Necklace Structure
نویسندگان
چکیده
منابع مشابه
Mobility of sodium dodecyl sulphate - protein complexes.
Reduced and unreduced lysozyme aggregates formed by formaldehyde cross-linking comprise a set of model compounds for studying the effects of protein conformation on the electrophoretic mobilities of sodium dodecyl sulphate-protein complexes. The reduced aggregates were indistinguisable from normal proteins, but the unreduced aggregates migrated anomalously fast by about 14%. Contrary to expecta...
متن کاملA small-angle X-ray scattering study of the structure of lysozyme-sodium dodecyl sulfate complexes.
The structure of lysozyme-sodium dodecyl sulfate (SDS) complexes in solution is studied using small-angle X-ray scattering (SAXS). The SAXS data cannot be explained by the necklace and bead model for unfolded polypeptide chain interspersed with surfactant micelles. For the protein and surfactant concentrations used in the study, there is only marginal growth of SDS micelles as they complex with...
متن کاملThe gross conformation of protein-sodium dodecyl sulfate complexes.
The interaction of sodium dodecyl sulfate with a wide variety of proteins is characterized by a high binding ratio when the monomer concentration of amphiphile exceeds 5 x 10W4 M. This binding ratio on a gram to gram basis is identical for all proteins investigated. The protein portion of the complex contains a high degree of order, and hydrodynamic studies suggest that the complex is a rodlike...
متن کاملMicrodisc gel electrophoresis in sodium dodecyl sulfate of organic material from rat otoconial complexes.
The gravity receptors of all vertebrates utilize a “test mass” consisting of a complex arrangement of mineral and organic substance that lies over the sensory receptor areas ( m a c u l a ~ ) . ~ ~ Carlstrom has shown that in most vertebrates, the mineral is a polymorph of calcium carbonate (vaterite, aragonite, calcite] in the form of minute, single crystals called otoconia (“ear dust”)? The o...
متن کاملSodium dodecyl sulphate electrophoresis of wheat gliadins.
Polypeptide chain weights of a-, 8and y-gliadin fractions have been estimated by polyacrylamide gel electrophoresis in the presence of mercaptoethanol and sodium dodecyl sulphate. Values lie in the range 32 000 to 44 OOO. When available data on the amino acid analyses were adjusted and compared, significant similarities emerged. It is suggested that these gliadins contain a minimum of 4 cystine...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 2008
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1995.0818a.x